Modified detergent Ziehl-Neelsen technique for the staining of Cyclospora cayetanensis.

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Modified detergent Ziehl-Neelsen technique for the staining of Cyclospora cayetanensis.

Cyclospora cayetanensis is a cause of prolonged diarrhoea, mainly in travellers. Laboratory diagnosis may be achieved by a number of methods such as the staining of faecal smears by the modified Ziehl-Neelsen (ZN) technique. Safer methods using this technique have been described for the staining of acid fast bacilli and cryptosporidia by replacing the phenol content of the carbol fuschin stain ...

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Ziehl-Neelsen Staining Technique Can Diagnose Paragonimiasis

BACKGROUND We evaluated the Ziehl-Neelsen staining (ZNS) technique for the diagnosis of paragonimiasis in Laos and compared different modifications of the ZNS techniques. METHODOLOGY WE APPLIED THE FOLLOWING APPROACH: We (1) examined a paragonimiasis index case's sputum with wet film direct examination (WF) and ZNS; (2) re-examined stored ZNS slides from two provinces; (3) compared prospectiv...

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Sub-optimal Specificity of Modified Ziehl-Neelsen Staining for Quick Identification of Tuberculous Meningitis

Background: Microbiological confirmation of tuberculous meningitis (TBM) remains problematic. We assessed the diagnostic performance of a modified Ziehl-Neelsen (MZN) staining method that showed promise in earlier studies. Methods: Patients evaluated for TBM in Shaanxi province, China, were prospectively enrolled from May, 2011 to April, 2013. Cerebrospinal fluid (CSF) specimens were evaluated ...

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A Comparison of Ziehl-Neelsen Staining and Fluorescent Microscopy for Diagnosis of Pulmonary Tuberculosis

According to WHO one third of the world population have tuberculosis. The present study was undertaken to compare the efficacy of flurochrome(FI) stain with conventional Z-N stain in the diagnosis of pulmonary & extrapulmonary tuberculosis. 388 cases of suspected pulmonary tuberculosis were included in the study. All samples were screen for Acid Fast Bacilli (AFB) by Z-N & Fluorescent staining ...

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Acriflavine as a Counter-Stain in the Ziehl-Neelsen Method of Staining

a strength of 1:500 with distilled water, and the process of staining is carried out exactly in the same way as that described under the above method, except that acriflavine in the abovementioned strength is used instead of methylene blue. I use the counter-stain for half to one minute. To obtain the best results, all the detail in the careful preparation and fixation of smears, as well as the...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1996

ISSN: 0021-9746

DOI: 10.1136/jcp.49.6.511